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SRX2505700: GSM2464150: 18_2B_HighLight_LowO2; Meiothermus ruber; Thermosynechococcus elongatus BP-1; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 20.9M spots, 1.6G bases, 800.7Mb downloads

Submitted by: NCBI (GEO)
Study: Analysis of Thermosynechococcus elongatus BP1 and Meiothermus ruber strain A co-cultured under variable light and oxygen conditions
show Abstracthide Abstract
The transcriptional response of the cyanobacteria T. elongatus to co-cultiavation with a heterotrophic partner M. ruber was examined under three varying light levels and three varying oxygen levels. Overall design: Three light levels were examined: low (197 µmol photons m-2 sec-1), mid (1190 µmol photons m-2 sec-1), and high (1995 µmol photons m-2 sec-1) all with oxygen at a partial pressure of zero . Three oxygen levels were also examined: low (oxygen at 7% of atmosphere), mid (oxygen at 150% of atmosphere) and high (oxygen at 217% of atmosphere) all at a light level of 197 µmol photons m-2 sec-1. T. elongatus was examined both axenically and with a hetertrophic partner of each of these six conditions. The high light condition overlaps with the low oxygen condition. Each condition used two biological replicates except for HighLight_LowO2 which used 4 biological replicates for a total of 12 data points
Sample: 18_2B_HighLight_LowO2
SAMN06242750 • SRS1930022 • All experiments • All runs
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted using Promega following by DNAseI treatment Template cDNA was prepared using the NuGen Ovation Universal RNAseq kit. Sequencing was carried using the Illumina NextSeq 500.
Experiment attributes:
GEO Accession: GSM2464150
Links:
Runs: 1 run, 20.9M spots, 1.6G bases, 800.7Mb
Run# of Spots# of BasesSizePublished
SRR518985920,928,3771.6G800.7Mb2017-01-23

ID:
3631319

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